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Immunization with a peptide mimicking lipoteichoic acid induces memory B cells in BALB/c mice.

Authors :
Yi, Xia-Yu
Hou, Xiao-Rui
Huang, Zhao-Xia
Zhu, Ping
Liu, Bei-Yi
Source :
BMC Infectious Diseases. 4/2/2024, Vol. 22 Issue 1, p1-10. 10p.
Publication Year :
2024

Abstract

Background: There is an urgent clinical need for developing novel immunoprophylaxis and immunotherapy strategies against Staphylococcus aureus (S. aureus). In our previous work, immunization with a tetra-branched multiple antigenic peptide, named MAP2-3 that mimics lipoteichoic acid, a cell wall component of S. aureus, successfully induced a humoral immune response and protected BALB/c mice against S. aureus systemic infection. In this study, we further investigated whether vaccination with MAP2-3 can elicit immunologic memory. Methods: BALB/c mice were immunized with MAP2-3 five times. After one month of the last vaccination, mice were challenged with heat-killed S. aureus via intraperitoneal injection. After a 7-day inoculation, the percentage of plasma cells, memory B cells, effector memory T cells, and follicular helper T cells were detected by flow cytometry. The levels of IL-6, IL-21, IL-2, and IFN-γ were measured by real-time PCR and ELISA. Flow cytometry results were compared by using one-way ANOVA or Mann-Whitney test, real-time PCR results were compared by using one-way ANOVA, and ELISA results were compared by using one-way ANOVA or student's t-test. Results: The percentage of plasma cells and memory B cells in the spleen and bone marrow from the MAP2-3 immunized mice was significantly higher than that from the control mice. The percentage of effector memory T cells in spleens and lymphoid nodes as well as follicular helper T cells in spleens from the MAP2-3 immunized mice were also higher. Moreover, the levels of IL-6 and IL-21, two critical cytokines for the development of memory B cells, were significantly higher in the isolated splenocytes from immunized mice after lipoteichoic acid stimulation. Conclusions: Immunization with MAP2-3 can efficiently induce memory B cells and memory T cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14712334
Volume :
22
Issue :
1
Database :
Academic Search Index
Journal :
BMC Infectious Diseases
Publication Type :
Academic Journal
Accession number :
176405988
Full Text :
https://doi.org/10.1186/s12879-024-09262-8