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A fluorescent probe for detecting H2O2 and delivering H2S in lysosomes and its application in maintaining the redox environments.
- Source :
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Talanta . Jun2024, Vol. 273, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
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Abstract
- Hydrogen peroxide (H 2 O 2) is a reactive oxygen species (ROS) that can be used as a marker for the occurrence of oxidative stress in the organism. Lysosomes serve as intracellular digestive sites, and when the concentration of H 2 O 2 in them is abnormal, lysosomal function is often impaired, leading to the development of diseases. Hydrogen sulfide (H 2 S) acts as a gaseous signaling molecule that scavenges H 2 O 2 from cells and tissues, thereby maintaining the redox environment of the body. However, most of the reported hydrogen peroxide fluorescent probes so far can only detect H 2 O 2 , but cannot maintain the intracellular redox environment. In this paper, an H 2 O 2 fluorescent probe LN-HOD with lysosomal targeting properties was designed and synthesized by combining the H 2 O 2 recognition site with a naphthylamine fluorophore via a thiocarbamate moiety. The probe has the advantages of large Stokes shift (110 nm), high sensitivity and good H 2 S release capability. The probe LN-HOD can be used to detect H 2 O 2 in cells, zebrafish and plant roots. In addition, LN-HOD detects changes in the concentration of H 2 O 2 in plant roots when Arabidopsis is stressed by cadmium ion (Cd2+). And through its ability to release H 2 S, it can help to remove excess H 2 O 2 and maintain the redox environment in cells, zebrafish and plant roots. The present work provides new ideas for the detection and assisted removal of H 2 O 2 , which contributes to the in-depth study of the cellular microenvironment in organisms. In this paper, we designed and synthesized LN-HOD , a fluorescent probe targeting the detection of H 2 O 2 in lysosomes, by combining the H 2 O 2 recognition site with a naphthalimide fluorophore via a thiocarbamate moiety. The probe has the advantages of large Stokes displacement, high sensitivity and good H 2 S release capability. The probe LN-HOD can be used to detect H 2 O 2 in cells, zebrafish and plant roots. In addition, LN-HOD detects changes in the concentration of H 2 O 2 in plant roots when Arabidopsis is stressed by cadmium ion (Cd2+). And through its ability to release H 2 S, it can help to remove excess H 2 O 2 and maintain the redox environment in cells, zebrafish and plant roots. The present work provides new ideas for the detection and assisted removal of H 2 O 2 , which contributes to the in-depth study of the cellular microenvironment in organisms. [Display omitted] • The probe LN-HOD not only detects H 2 O 2 but also releases H 2 S. • LN-HOD has the ability to release H 2 S in cells, zebrafish and Arabidopsis. • LN-HOD has a good ability to maintain the redox environment in cells. • LN-HOD can detect changes in H 2 O 2 concentration in plants induced by Cd2+ ion stress effects. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 273
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 176467395
- Full Text :
- https://doi.org/10.1016/j.talanta.2024.125894