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Analysis of peripheral immune cell typing in breast cancer lung metastasis model of miR-155 knockout mice.
- Source :
-
Chinese Journal of Immunology . Mar2024, Vol. 40 Issue 3, p484-490. 7p. - Publication Year :
- 2024
-
Abstract
- Objective: To establish a mouse model of breast cancer lung metastasis with miR-155 knockout (miR155-/-) mice, and to compare the difference of peripheral blood immune cell typing between miR155-/- mice and C57BL/6J wide-type (WT) mice. Methods: Bioinformatics analysis was used to explore the expression level of miR-155 in breast cancer tissues and peripheral serum, and its relationship with prognosis. Mouse model of lung metastasis of breast cancer was established by tail vein injection; peripheral blood was collected for flow cytometry, and the immune cell typing was analyzed; the lung tissues were collected for immunohisto-chemical detection to observe the tumor metastasis. Results: Percentage of T lymphocytes and monocytes in peripheral blood of miR155-/- mice was significantly decreased compared with WT mice (P<0.05), percentage of myeloid inhibitory cells (MDSCs) was increased significantly (P<0.05), in which the proportion of monocyte subsets (M-MDSC) was significantly decreased (P<0.05), while the proportion of granulocyte subsets (G-MDSC) was significantly increased (P<0.05). In lung metastasis model of breast cancer, percentage of T lymphocytes in peripheral blood of miR155-/- mice was significantly higher compared with WT mice, while percentage of NK cells was decreased significantly (P<0.05), percentage of neutrophil was significantly decreased (P<0.001), proportion of Th cells in T lymphocytes was significantly decreased (P<0.05), proportion of M-MDSCs was significantly decreased (P< 0.01), while proportion of G-MDSCs was significantly increased (P<0.01). Conclusion: Deletion of miR-155 gene leads to significant differences in peripheral immune cell typing, making mice more susceptible to lung metastasis of breast cancer. [ABSTRACT FROM AUTHOR]
- Subjects :
- *METASTATIC breast cancer
*KNOCKOUT mice
*T cells
*KILLER cells
*MYELOID cells
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000484X
- Volume :
- 40
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Chinese Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 176518355
- Full Text :
- https://doi.org/10.3969/j.issn.1000-484X.2024.03.007