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Eber Gölü Prokaryotik Çeşitliliğinin Metagenomik Çalışmasıyla Karakterizasyonu.
- Source :
-
Journal of the Institute of Science & Technology / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi . Mar2024, Vol. 14 Issue 1, p437-446. 10p. - Publication Year :
- 2024
-
Abstract
- Eber Lake is located on the migration route of birds in Turkey and provides natural breeding and habitat for many bird species thanks to its reed areas. Despite being such an important lake in our country, there has been insufficient research on prokaryotic diversity. Metagenomic analysis enables the determination of the species, genetic structures, functional roles, and their effects on human and ecological health of microorganisms obtained through DNA isolation. Next-generation sequencing (NGS) is a modern DNA/RNA sequencing technology that allows for the simultaneous high-speed and high-precision sequencing of many DNA or RNA samples. In this study, we used high-throughput NGS methods for metagenomic analysis of 16S ribosomal DNA (rDNA) (V3-V4 regions) sequences from Eber Lake. The obtained sequence data using Illumina NovaSeq technology was analyzed with MOTHUR software to investigate prokaryotic diversity. The analysis revealed that the Proteobacteria, Verrucomicrobia, Bacteroidetes, and Actinobacteria phyla were dominant, and the prokaryotic diversity in Eber Lake was characterized in detail. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Turkish
- ISSN :
- 21460574
- Volume :
- 14
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of the Institute of Science & Technology / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi
- Publication Type :
- Academic Journal
- Accession number :
- 176522958
- Full Text :
- https://doi.org/10.21597/jist.1265800