Back to Search Start Over

Eber Gölü Prokaryotik Çeşitliliğinin Metagenomik Çalışmasıyla Karakterizasyonu.

Authors :
PAT, Fahri
FİDAN PEDÜK, Sultan
AKÇAY, Neşe
KIZIL PAT, Hatice Kübra
ARICAN, Ercan
Source :
Journal of the Institute of Science & Technology / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi. Mar2024, Vol. 14 Issue 1, p437-446. 10p.
Publication Year :
2024

Abstract

Eber Lake is located on the migration route of birds in Turkey and provides natural breeding and habitat for many bird species thanks to its reed areas. Despite being such an important lake in our country, there has been insufficient research on prokaryotic diversity. Metagenomic analysis enables the determination of the species, genetic structures, functional roles, and their effects on human and ecological health of microorganisms obtained through DNA isolation. Next-generation sequencing (NGS) is a modern DNA/RNA sequencing technology that allows for the simultaneous high-speed and high-precision sequencing of many DNA or RNA samples. In this study, we used high-throughput NGS methods for metagenomic analysis of 16S ribosomal DNA (rDNA) (V3-V4 regions) sequences from Eber Lake. The obtained sequence data using Illumina NovaSeq technology was analyzed with MOTHUR software to investigate prokaryotic diversity. The analysis revealed that the Proteobacteria, Verrucomicrobia, Bacteroidetes, and Actinobacteria phyla were dominant, and the prokaryotic diversity in Eber Lake was characterized in detail. [ABSTRACT FROM AUTHOR]

Details

Language :
Turkish
ISSN :
21460574
Volume :
14
Issue :
1
Database :
Academic Search Index
Journal :
Journal of the Institute of Science & Technology / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi
Publication Type :
Academic Journal
Accession number :
176522958
Full Text :
https://doi.org/10.21597/jist.1265800