平凉刺五加叶生药学研究及其有效成分含量测定.

Qualitative analysis of A. senticosus plants in Pingliang was conducted using trait identification and microscopic identification methods, and the content of active ingredients in the leaves of A. senticosus in Pingliang was determined using high-performance liquid chromatography. Trait identification showed that the leaves of A. senticosus in Pingliang were mostly elliptic obovate or oblong, the base was broadly wedged, the edge was heavily serrated, and the leaves had special aroma. Microscopic identification showed that the powder of A. senticosus leaves in Pingliang contained many amorphous pores, threaded conduits, calcium oxalate clusters, fibers and so on. The regression equations of eleutheroside B and eleutheroside E had a good linear relationship, and the RSD of precision, stability and repeatability tests were all less than 5.00%. Among the three extraction methods, microwave ultrasonic-assisted extraction had the highest content of active components, the content of eleutheroside B and eleutheroside E was 0.085% and 0.358%, respectively; microwave extraction method followed, with contents of 0.075% and 0.328%, respectively; the ultrasonic-assisted extraction method had the lowest content, which was 0.054% and 0.222%, respectively. [ABSTRACT FROM AUTHOR]