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Impairment of Listeria monocytogenes biofilm developed on industrial surfaces by Latilactobacillus curvatus CRL1579 bacteriocin.
- Source :
-
Food Microbiology . Aug2024, Vol. 121, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- The effect of lactocin AL705, bacteriocin produced by Latilactobacillus (Lat.) curvatus CRL1579 against Listeria biofilms on stainless steel (SS) and polytetrafluoroethylene (PTFE) coupons at 10 °C was investigated. L. monocytogenes FBUNT showed the greatest adhesion on both surfaces associated to the hydrophobicity of cell surface. Partially purified bacteriocin (800 UA/mL) effectively inhibited L. monocytogenes preformed biofilm through displacement strategy, reducing the pathogen by 5.54 ± 0.26 and 4.74 ± 0.05 log cycles at 3 and 6 days, respectively. The bacteriocin-producer decreased the pathogen biofilm by ∼2.84 log cycles. Control and Bac− treated samples reached cell counts of 7.05 ± 0.18 and 6.79 ± 0.06 log CFU/cm2 after 6 days of incubation. Confocal scanning laser microscopy (CLSM) allowed visualizing the inhibitory effect of lactocin AL705 on L. monocytogenes preformed biofilms under static and hydrodynamic flow conditions. A greater effect of the bacteriocin was found at 3 days independently of the surface matrix and pathogen growth conditions at 10 °C. As a more realistic approach, biofilm displacement strategy under continuous flow conditions showed a significant loss of biomass, mean thickness and substratum coverage of pathogen biofilm. These findings highlight the anti-biofilm capacity of lactocin AL705 and their potential application in food industries. • Lactocin AL705 inhibited L. monocytogenes biofilm through displacement strategy. • The greatest effectiveness was found on SS and PTFE surfaces after 3 days at 10 °C. • Bacteriocin reduced of biofilm biomass and thickness in continuous flow conditions. • Lactocin AL705 proved to be an effective and promising anti-biofilm agent. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LISTERIA monocytogenes
*BIOFILMS
*LASER microscopy
*STAINLESS steel
Subjects
Details
- Language :
- English
- ISSN :
- 07400020
- Volume :
- 121
- Database :
- Academic Search Index
- Journal :
- Food Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 176632168
- Full Text :
- https://doi.org/10.1016/j.fm.2024.104491