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Hemoglobin-inorganic hybrid nanoflowers: Synthesis and applications for carbene N–H insertion reaction.
- Source :
-
Biocatalysis & Biotransformation . Jun2024, Vol. 42 Issue 3, p308-315. 8p. - Publication Year :
- 2024
-
Abstract
- Hemoglobin (Hb) is a hemoprotein containing iron porphyrin, which can catalyse a variety of unnatural reactions. Due to its poor reusability and stability, in this study, we developed a new method to prepare Hb-Cu3(PO4)2 hybrid nanoflowers (HNFs) by co-incubating Hb with Cu2+. This immobilized Hb was used to catalyse the synthesis of ethyl N-phenylglycinate via the carbene N–H insertion reaction. Under the optimum reaction conditions (aniline 1 mmol; ethyl diazoacetate 1 mmol; deionized (DI) water 10 ml; catalyst (protein content: 15 mg); sodium hydrosulfite 25 mg; 25 °C; 12 h), the catalytic yield of Hb-Cu3(PO4)2 HNFs reached as high as 87% and that of free Hb was 74%. In addition, Hb-Cu3(PO4)2 HNFs can maintain a high catalytic performance after 15 cycles and still achieve yields close to 70% of the initial yield after 60 days of storage, indicating that Hb-Cu3(PO4)2 HNFs have a high potential for application in Hb-catalysed organic reactions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10242422
- Volume :
- 42
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biocatalysis & Biotransformation
- Publication Type :
- Academic Journal
- Accession number :
- 176635170
- Full Text :
- https://doi.org/10.1080/10242422.2023.2195036