慢性静力性损伤模型家兔颈后肌群转录组学的 生物信息分析.

Objective：Transcriptome sequencing analysis was used to study the differentially expressed genes （DEGs） and key signaling pathways of the posterior cervical muscles of rabbits in a model of chronic static injury. Method：The chronic static load injury model of posterior cervical muscle in rabbits was replicated by longterm repeated flexion of the neck of rabbits. DEGs were screened by transcriptome sequencing and analyzed for GO function enrichment and KEGG pathway enrichment. Build a PPI network and filter out the top 10 DEGs. Result：Compared with the blank group，there were 310 DEGs，of which 193 were upward and 117 were downward in the model group. GO analysis showed that the different genes in the model group were mainly involved in related functions such as inflammation and immune response regulation. KEGG analysis showed that phagosomes were active in Fcγ-R-mediated phagocytosis and NF-κB. The PPI network identified 10 hub genes，among which TLR4，CTSS，ITGB2，TYROBP，CD74，LAPTM5 and other expressions were up-regulated， and only PPARG expression was down-regulated. Conclusion：The pathological changes of chronic static injury of cervical muscles involve plenty of basic biological processes，and the inflammatory response and the disorder of immune microenvironment play an important role in it. Transcriptional activation of differential genes closely related to inflammatory responses and pathway molecules such as NF-κB signaling pathway，phagosomes，and Fcγ-R-mediated phagocytosis may be its main molecular mechanisms. [ABSTRACT FROM AUTHOR]