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A phenothiazine coumarin based fluorescent probe with AIE feature for real-time detecting lysosomal ClO– in living cell.
- Source :
-
Microchemical Journal . May2024, Vol. 200, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- [Display omitted] • A phenothiazine coumarin based fluorescent probe (WX-Lyso) for the detection of ClO– was synthesized. • WX-Lyso displayed a low LOD (0.99 µm) and rapid responsive time to ClO– (<5 s). • WX-Lyso was proved successfully has good lysosomal-targeting and imaging abilities to ClO– in Hela cells. A new phenothiazine coumarin based probe WX-Lyso for detection of ClO– was developed. The probe showed obvious aggregation-induced emission (AIE) characteristic and exhibited a highly selective and sensitive turn-on fluorescence signal at 544 nm for ClO– with a large Stokes shift of 144 nm. The limit of detection (LOD) of ClO– was measured as low as 0.99 μM and a good line equation was obtained in the 0–50 μM concentration range. The detection mechanism was elucidated FT-IR, ESI-MS analysis and DFT. Furthermore, the probe had been demonstrated successfully has good lysosomal-targeting and high-contrast imaging abilities, which has the potential to become a new chemical tool and provide help for detecting ClO– in living systems. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0026265X
- Volume :
- 200
- Database :
- Academic Search Index
- Journal :
- Microchemical Journal
- Publication Type :
- Academic Journal
- Accession number :
- 176648114
- Full Text :
- https://doi.org/10.1016/j.microc.2024.110268