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Cloning and spatio-temporal expression of CsKr-h1 encoding the juvenile hormone response gene in Coccinella septempunctata L.
- Source :
-
Bulletin of Entomological Research . Feb2024, Vol. 114 Issue 1, p99-106. 8p. - Publication Year :
- 2024
-
Abstract
- The gene encoding juvenile hormone response (Krüppel homolog1, Kr-hl) in Coccinella septempunctata was investigated by cloning and analysing expression profiles in different developmental stages and tissues by quantitative real-time polymerase chain reaction (PCR). C. septempunctata Kr-hl (CsKr-hl) encoded a 1338 bp open reading frame (ORF) with a predicted protein product of 445 amino acids; the latter showed high similarity to orthologs in other species and contained eight highly-conserved Zn-finger motifs for DNA-binding. CsKr-hl was expressed in different developmental stages of C. septempunctata. The expression levels of CsKr-hl in eggs, 2nd, 3rd, 4th instar larvae, and pupa were 3.31, 2.30, 7.09, 0.58, and 7.48 times the number of 1st instar larvae, respectively. CsKr-hl expression levels in female adults gradually increased at 25–30 days and were significantly higher than expression at 1–20 days. CsKr-hl expression in 20–30 days-old male adults was significantly higher than males aged 1–15 days. CsKr-hl expression levels in heads of male and female adults were significantly higher than expression levels in the thorax, adipose, and reproductive system. Interestingly, CsKr-hl expression levels in the adipose and reproductive system of female adults were significantly higher than in adult male corresponding organs, which suggest that CsKr-hl plays an important role in regulating reproductive development in C. septempunctata. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00074853
- Volume :
- 114
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Bulletin of Entomological Research
- Publication Type :
- Academic Journal
- Accession number :
- 176652477
- Full Text :
- https://doi.org/10.1017/S0007485323000652