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Enhanced development of human pluripotent stem cell-derived cerebral organoids via an electrical stimulation bioreactor.

Authors :
Najar-Asl, Mostafa
Halvaei, Majid
Abolhasani, Rouhollah
Mirsadeghi, Sara
Simorgh, Susan
Rahmani, Saeid
Pooyan, Paria
Yektadoost, Elham
Kiani, Sahar
Abolghasemi-Dehaqani, Mohammad-Reza
Javan, Mohammad
Kazemi Ashtiani, Mohammad
Baharvand, Hossein
Source :
Chemical Engineering Journal. May2024, Vol. 487, pN.PAG-N.PAG. 1p.
Publication Year :
2024

Abstract

• ES of human COs promotes proliferation of neural progenitor cells. • ES increases neurogenesis, function and network formation in human COs. • ES improves CO cortical plate organization into upper and deep layers. Human brain-like 3D cultures or brain organoids are promising models for expanding our knowledge of brain development and the mechanisms that contribute to neurological disorders. Moreover, it may address the hardware bottleneck for the fast-growing field of artificial intelligence and the development of brain-machine interfaces. Current methods for developing brain organoids are limited mainly due to insufficient differentiation and inaccurate structural organization. Here, a novel engineering approach is proposed to accelerates the development of human cerebral organoids by electrical stimulation via an electrical bioreactor. This approach not only promotes proliferation and neurogenesis but also improves function, network formation, and organization of the cortical plate into upper and deep layers in which PI3K/Akt pathway at least partially participates. These results demonstrate the impact of electrical stimulation on generation of more mature and the functional cerebral organoids and may provide new insights into the hardware of its applications. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13858947
Volume :
487
Database :
Academic Search Index
Journal :
Chemical Engineering Journal
Publication Type :
Academic Journal
Accession number :
176759700
Full Text :
https://doi.org/10.1016/j.cej.2024.150368