Effect of Ginsenoside Rd on the Biological Characteristics of Lung Adenocarcinoma H1299 Cell Line.

To explore the effect of ginsenoside Rd on the biological characteristics of lung adenocarcinoma H1299 cell line. Firstly, 10 µmol/l ginsenoside Rd, 20 µmol/l ginsenoside Rd, 40 µmol/l ginsenoside Rd, 80 µmol/l ginsenoside Rd, 160 µmol/l ginsenoside Rd were applied to treat H1299 cell line. 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay was used to measure the inhibitory rate of glutathione reductase on cell viability, and the half-maximal inhibitory concentration value was calculated as 48.17 µmol/l. After treating H1299 cell line with 48.17 µmol/l ginsenoside Rd for 3 d, effects of ginsenoside Rd on proliferation ability, migration ability, and apoptosis were respectively detected using Ki67 immunofluorescence, Transwell culture system, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay kit. Ginsenoside Rd had inhibitory effects on the viability of H1299 cells, and the inhibition is concentration-dependent. The half-maximal inhibitory concentration value is 131.92 µmol/l; compared to the control group, treatment of H1299 cells with 130 µmol/l ginsenoside Rd induced a significant decrease in Ki67-positive cells number and migratory cells. However, there was hardly any observed apoptosis in both groups. Ginsenoside Rd can suppress activity, proliferation ability, and migration capability of H1299 cell line, which indicates a noticeable suppression of malignancy in H1299 cells. Ginsenoside Rd may have potential therapeutic effects in the tr eatment of lung adenocarcinoma. [ABSTRACT FROM AUTHOR]