MAPK improves intestional obstruction in pregnancy through inhibiting TLR4/Myd88/NF-κB pathway.

Objective To investigate the effect of MAPK on intestinal obstruction during pregnancy. Methods A total of 105 SPF Wistar rats (female = 70 and male = 35) were selected. After placing all the rats in the same cage, 33 female rats were pregnant, and 8 rats were assigned to the control group and the other 25 rats were used to established bowel obstruction model. A total of 24 rats were successfully modelled, which were divided into model group (n = 8), up-regulation group (n = 8), and down-regulation group (n = 8). Results Compared with the model group, MAPK expression, VIP, GAS, TNF-α, IL-6 levels, TLR4, Myd88, NF-κB mRNA were increased and MOT, IL-4, IL-10 levels were decreased in up-regulation group; MAPK expression, VIP, GAS, TNF-α, IL-6 levels, TLR4, Myd88, NF-κB mRNA were decreased and MOT, IL-4, IL-10 levels were increased in down-regulation group (P < 0.05). Conclusion MAPK down-regulation reduced inflammatory response and improved bowel obstruction in pregnancy, and the mechanism may be related to the inhibition of TLR4/Myd88/NF-κB pathway. [ABSTRACT FROM AUTHOR]