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Lessons on harmonization of scoring criteria for dicentric chromosome assay in South Korea.
- Source :
-
International Journal of Radiation Biology . 2024, Vol. 100 Issue 5, p709-714. 6p. - Publication Year :
- 2024
-
Abstract
- Networking with other biodosimetry laboratories is necessary to assess the radiation exposure of many individuals in large-scale radiological accidents. The Korea biodosimetry network, K-BioDos, prepared harmonized scoring guidelines for dicentric chromosome assay to obtain homogeneous results within the network and investigated the efficiency of the guidelines. Three laboratories in K-BioDos harmonized the scoring guidelines for dicentric chromosome assay. The results of scoring dicentric chromosomes using the harmonized scoring guidelines were compared with the laboratories' results using their own methods. Feedback was collected from the scorers following the three intercomparison exercises in 3 consecutive years. K-BioDos members showed comparable capacity to score dicentrics in the three exercises. However, the results of the K-BioDos guidelines showed no significant improvement over those of the scorers' own methods. According to the scorers, our harmonized guidelines led to more rejected metaphases and ultimately decreased the number of scorable metaphases compared with their own methods. Moreover, the scoring time was sometimes longer with the K-BioDos protocol because some scorers were not yet familiar with the guidelines, though most scorers reported that the time decreased or was unchanged. These challenges may cause low adherence to the guidelines. Most scorers expressed willingness to use the guidelines to select scorable metaphases or identify dicentrics for other biodosimetry works, whereas one did not want to use it due to the difference from their calibration curves. We identified potential resistance to following the harmonized guidelines and received requests for more detailed methods. Our findings suggest that the harmonized criteria should be continually updated, and education and training should be provided for all scorers. These changes could allow members within the biodosimetry network to successfully collaborate and support each other in large-scale radiological accidents. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CHROMOSOMES
*RADIATION exposure
Subjects
Details
- Language :
- English
- ISSN :
- 09553002
- Volume :
- 100
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- International Journal of Radiation Biology
- Publication Type :
- Academic Journal
- Accession number :
- 176934041
- Full Text :
- https://doi.org/10.1080/09553002.2024.2316603