单病毒示踪技术在动植物细胞中的应用进展.

Virus is a kind of simple microorganism specializing in intracellular parasitics, which is one of the main pathogens causing animal and plant diseases. The development of single-virus tracking（SVT）technology makes it possible to reveal the fine process of viral life activity. SVT is a new method to study the mechanism of viral infection based on fluorescence labeling and high spatio-temporal resolution imaging, which allows the observation of the life activity of a single or multiple viruses. In recent years, the SVT technology has made great progress in the study of virus entry, replication, and intercellular transmission, providing new insights into the mechanism of virus infection. In this paper, the principle and strategy types of virus labeling are briefly introduced, and the research progress of virus fluorescence labeling in single virus tracing is summarized, especially the labeling and imaging methods of different structures of viruses, aiming to provide new ideas for optimizing labeling strategies and solving the problem of efficient labeling of different types and structures of viruses. [ABSTRACT FROM AUTHOR]