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A protoplast-based transient gene expression assay for the identification of heat and oxidative stress-regulatory genes in perennial ryegrass.
- Source :
-
Plant Methods . 5/9/2024, Vol. 20 Issue 1, p1-9. 9p. - Publication Year :
- 2024
-
Abstract
- Background: With the accumulating omics data, an efficient and time-saving transient assay to express target genes is desired. Mesophyll protoplasts, maintaining most stress-physiological responses and cellular activities as intact plants, offer an alternative transient assay to study target genes' effects on heat and oxidative stress responses. Results: In this study, a perennial ryegrass (Loliumperenne L.) mesophyll protoplast-based assay was established to effectively over- or down-regulate target genes. The relative expression levels of the target genes could be quantified using RT-qPCR, and the effects of heat and H2O2-induced oxidative stress on protoplasts' viability could be quantitatively measured. The practicality of the assay was demonstrated by identifying the potential thermos-sensor genes LpTT3.1/LpTT3.2 in ryegrass that over-expressing these genes significantly altered protoplasts' viability rates after heat stress. Conclusion: This protoplast-based rapid stress regulatory gene identification assay was briefed as 'PRIDA' that will complement the stable genetic transformation studies to rapidly identify candidate stress-regulatory genes in perennial ryegrass and other grass species. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17464811
- Volume :
- 20
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Plant Methods
- Publication Type :
- Academic Journal
- Accession number :
- 177193722
- Full Text :
- https://doi.org/10.1186/s13007-024-01192-5