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Discovery and characterization of sgRNA-sequence-independent DNA cleavage from CRISPR/Cas9 in mouse embryos.
- Source :
-
Genomics . May2024, Vol. 116 Issue 3, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- The CRISPR/Cas9 system can induce off-target effects in programmed gene editing, but there have been few reports on cleavage detection and their affection in embryo development. To study these events, sgRNAs with different off-target rates were designed and compared after micro-injected into mouse zygotes, and γH2AX was used for DNA cleavage sites analysis by immunostaining and CUT&Tag. Although the low off-target sgRNA were usually selected for production gene editing animals, γH2AX immunofluorescence indicated that there was a relative DSB peak at 15 h after Cas9 system injection, and the number of γH2AX foci at the peak was significantly higher in the low off-target sgRNA-injected group than in the control group. Further, the result of CUT&Tag sequencing analysis showed more double-strand breaks (DSBs) related sequences were detected in low off-target sgRNA-injected group than control and the distribution of DSB related sequences had no chromosome specificity. Gene Ontology (GO) annotation analysis of the DSB related sequences showed that these sequences were mainly concentrated at genes associated with some important biological processes, molecular functions, and cell components. In a conclusion, there are many sgRNA-sequence-independent DSBs in early mouse embryos when the Cas9 system is used for gene editing and the DSB related sequence could be detected and characterized in the genome. These results and method should also be considered in using or optimizing the Cas9 system. • There was a relative DSB peak at 15 h after Cas9 system injection. • More DSBs related sequences were detected in low off-target sgRNA-injected embryos than control. • The DSB related sequences were mainly concentrated at genes associated with some important biological events. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CRISPRS
*EMBRYOS
*GENOME editing
*DNA
*MICE
Subjects
Details
- Language :
- English
- ISSN :
- 08887543
- Volume :
- 116
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 177394065
- Full Text :
- https://doi.org/10.1016/j.ygeno.2024.110836