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Evaluation of the Treatment Effects of Conditioned Medium from Human Orbital Adipose-Derived Stem Cells in a Corneal Alkali Burn Rabbit Model.
- Source :
-
Journal of Ocular Pharmacology & Therapeutics . May2024, Vol. 40 Issue 4, p222-231. 10p. - Publication Year :
- 2024
-
Abstract
- Purpose: This study aimed to evaluate the effects of a new treatment—conditioned medium from human orbital adipose-derived stem cells (OASC-CM)—on corneal recovery after alkali burns in a rabbit model. Methods: The corneal alkali burn rabbit model was established and treated with OASC-CM, conditioned medium from human abdominal subcutaneous adipose-derived stem cells (ABASC-CM), and fresh control culture medium (con-CM) three times a day for 7 days, respectively. Subsequently, the treatment effects were evaluated and compared through clinical, histological, immunohistochemical, and cytokine evaluations. Results: Clinically, OASC-CM alleviated corneal opacity and edema and promoted recovery of corneal epithelium defect. Histologically and immunohistochemically, OASC-CM inhibited neovascularization, conjunctivalization, and immuno-inflammatory reaction, while promoting corneal regeneration and rearrangement. Increased secretion of interleukin-10 and inhibited protein levels of cluster of differentiation 45, interferon-γ, and tumor necrosis factor-α were observed in the alkali-burned cornea after OASC-CM treatment, which might be the relevant molecular mechanism. Conclusions: OASC-CM showed significant effects on the recovery of rabbit corneal alkali burns and eliminated immunological and ethical limitations, representing a new option for corneal wound treatment. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10807683
- Volume :
- 40
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Ocular Pharmacology & Therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 177399836
- Full Text :
- https://doi.org/10.1089/jop.2023.0154