康乃馨抗尖孢镰刀菌无性系诱变技术.

Carnation wilt disease is one of the most serious fungal diseases in the planting process of fresh cut flowers of carnations. It has been reported that this disease is caused by Fusarium oxysporum f. sp. dianthi. Breeding and rational utilization of resistant varieties is still one of the most effective methods to control carnation Fusarium wilt. Using somaclonal variation and in vitro screening of toxins can cultivate disease-resistant breeding materials of carnations, accelerate the breeding process of disease resistant varieties, and provide new ideas for disease resistant breeding for carnations. To obtain breeding intermediate materials with resistance to wilt disease of carnations, in this study, tissue cultures derived from in vitro plantlets of spray carnation Purple Butterfly, which is susceptible to F. oxysporum f. sp. dianthi, were successfully used for induction of callus tissue and establishment of a suspension culture system through suspension culture. Resistant cell lines were screened by adding crude extract of F. oxysporum toxin after mutagenesis with ethyl methanesulfonate (EMS). The results showed that the optimal culture medium for inducing callus tissue was Murashig-Skoog medium+ 1.0 mg/L of dicamba, the screened optimal EMS combination was 0.4% and 4.0 hours of treatment. The appropriate selection pressure for screening carnation clones resistant to F. oxysporum was cultivation on 80.0% of crude toxin medium for 10 days. The optimal combination of hormones for induction of regenerated plants of carnations was 0.5 mg/L of 6-benzylaminopurine (BA) + 0.1 mg/L of thidiazuron (TDZ) + 0.1 mg/L of 1-naphthaleneacetic acid(NAA). Disease resistance identification by artificial inoculation with F. oxysporum found that the disease index of the disease resistant clone of Purple Butterfly was 45, indicating it was at medium-resistance level. [ABSTRACT FROM AUTHOR]