Back to Search
Start Over
Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917.
- Source :
-
Synthetic & Systems Biotechnology . Mar2024, Vol. 9 Issue 1, p165-175. 11p. - Publication Year :
- 2024
-
Abstract
- The probiotic bacterium Escherichia coli Nissle 1917 (EcN) holds significant promise for use in clinical and biological industries. However, the reliance on antibiotics to maintain plasmid-borne genes has overshadowed its benefits. In this study, we addressed this issue by engineering the endogenous cryptic plasmids pMUT1 and pMUT2. The non-essential elements were removed to create more stable derivatives pMUT1NR△ and pMUT2HBC△. Synthetic promoters by integrating binding motifs on sigma factors were further constructed and applied for expression of Bacteroides thetaiotaomicron heparinase III and the biosynthesis of ectoine. Compared to traditional antibiotic-dependent expression systems, our newly constructed antibiotic-free expression systems offer considerable advantages for clinical and synthetic biology applications. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PLASMIDS
*ANTIBIOTICS
*BACTEROIDES
*BIOSYNTHESIS
Subjects
Details
- Language :
- English
- ISSN :
- 20971206
- Volume :
- 9
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Synthetic & Systems Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 177450185
- Full Text :
- https://doi.org/10.1016/j.synbio.2024.01.006