碳酸锂联合长春新碱促进儿童急性 T 淋巴细胞白血病细胞增殖抑制和凋亡.

T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive tumor, in which T precursor cells undergo malignant transformation, and the side effects and drug resistance of chemotherapy drugs are still difficult problems hindering the success of treatment. Vincristine (VCR) is a traditional chemotherapy drug for children with significant efficacy in T-ALL, but its side effects are obvious. Lithium carbonate (Li2CO3) can enhance the efficacy of other chemotherapy drugs, but studies of its combination with VCR have not been reported. The purpose of this study is to investigate the effects of Li2CO3 combined with VCR on the proliferation, apoptosis and cell cycle of two pediatric T-ALL cell lines, CCRF-CEM and Jurkat cells. The results of thiazolyl blue tetrazolium bromide (MTT) colorimetric assay showed that the viability of both cells decreased with the increase of the concentration of VCR or Li2CO3 alone, which compared with the control group (P<0.05). The survival rates of the two kinds of cells were different under the same Li2CO3 concentration (P<0.01). Compared with the VCR group, the cell viability and half maximal inhibitory concentration (IC50) values of Li2CO3 combined with VCR group were reduced in the two kinds of cells, and the coefficient of drug interaction (CDI) between Li2CO3 and VCR was less than 1. Flow cytometry showed Li2CO3 combined with VCR group had the highest proportion of G2/M and apoptotic cells of the two kinds of cells among the control, Li2CO3, VCR and, Li2CO3 combined with VCR group. And there were significant differences between Li2CO3 combined with VCR group and VCR group (P<0.05). In conclusion, our experimental results suggest that the combination of Li2CO3 and VCR inhibits of T-ALL cell proliferation, arrests the cell cycle in the G2/M phase and increases cell apoptosis, which provides a new experimental basis for the clinical treatment of pediatric T-ALL, the reduction of VCR side effects, and also new ideas for drug development. [ABSTRACT FROM AUTHOR]