Back to Search Start Over

Progressive expansion of albumin adducts for organophosphorus nerve agent traceability based on single and group adduct collection.

Authors :
Wang, Jin
Lu, Xiaogang
Gao, Runli
Pei, Chengxin
Wang, Hongmei
Source :
Analytical & Bioanalytical Chemistry. Jun2024, Vol. 416 Issue 15, p3569-3584. 16p.
Publication Year :
2024

Abstract

Protein adducts are important biological targets for traceability of organophosphorus nerve agents (OPNAs). Currently, the recognized biomarkers that can be used in actual samples in the field of chemical forensics only include Y411 in albumin and the active nonapeptide in butyrylcholinesterase (BChE). To explore stable and reliable protein adducts and increase the accuracy of OPNAs traceability further, we gradually expanded OPNAs–albumin adducts based on single and group adduct collection. Several stable peptides were found via LC–MS/MS analysis in human serum albumin (HSA) exposed to OPNAs in a large exposure range. These adducts were present in HSA samples exposed to OPNAs of each concentration, which provided data support for the reliability and stability of using adducts to trace OPNAs. Meanwhile, the formation mechanism of OPNAs–cysteine adduct was clarified via computer simulations. Then, these active sites found and modified peptides were used as raw materials for progressive expansion of albumin adducts. We constructed an OPNAs–HSA adducts group, in which a specific agent is the exposure source, and three or more active peptides constitute data sets for OPNAs traceability. Compared with single or scattered protein adducts, the OPNAs–HSA adduct group improves OPNAs identification by mutual verification using active peptides or by narrowing the identity range of the exposure source. We also determined the minimum detectable concentration of OPNAs for the adduct group. Two or more peptides can be detected when there is an exposure of 50 times the molar excess of OPNAs in relation to HSA. This improved the accuracy of OPNAs exposure and identity confirmation. A collection of OPNAs–albumin adducts was also examined. The collection was established by collecting, classifying, and integrating the existing albumin adducts according to the species to which each albumin belongs, the types of agents, and protease. This method can serve as a reference for discovering new albumin adducts, characteristic phosphonylated peptides, and potential biomarkers. In addition, to avoid a false negative for OPNAs traceability using albumin adducts, we explored OPNAs–cholinesterase adducts because cholinesterase is more reactive with OPNAs than albumin. Seven active peptides in red blood cell acetylcholinesterase (RBC AChE) and serum BChE can assist in OPNAs exposure and identity confirmation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16182642
Volume :
416
Issue :
15
Database :
Academic Search Index
Journal :
Analytical & Bioanalytical Chemistry
Publication Type :
Academic Journal
Accession number :
177714250
Full Text :
https://doi.org/10.1007/s00216-024-05311-y