Splitting study of mechanism of Jianpi-Huazhi pill in treatment of ulcerative colitis based on TLR-4/MyD88/NF-κB pathway.

Objective: To establish ulcerative colitis (UC) rat model of spleen deficiency and dampness, and to explore effect of Jianpi-Huazhu pill and its different deconditions on TLR-4/MyD88/NF-κB pathway. Methods: A total of 64 Wistar rats were randomly divided into normal control group (n=8) and model group (n=56). UC rat model was established by traditional Chinese medicine syndrome combined with ethanol-2, 4, 6-trinitrobenzene sulfonic acid enema method. After successfully modeling, rats were randomly divided into model group (M), Mesalazine group (A), Jianpi-Huazhi pill group (B), Jianpi-Qinghua decoction group (C), Jianpi-Qinghua-Huoxue decoction group (D), Jianpi-Qinghua-Daozhi decoction group (E) and Qinghua-Daozhi-Huoxue decoction group (F), with 8 rats in each group, given corresponding drugs by gavage for 4 weeks. At end of course of treatment, all rats were sacrificed and samples were taken. Changes of body weight, disease activity and pathological changes of colon were observed. TNF-α expression in colon tissue was detected by ELISA and immunohistochemistry. RT-PCR and Western blot were used to detect mRNA and protein expressions of TLR-4, MyD88 and NF-κB in rat colon tissue. Results: Rats weight in Jianpi-Huazhi pill group, Jianpi- Qinghua decoction group, Jianpi-Qinghua-Huoxue decoction group and Jianpi-Qinghua-Daozhi decoction group were obviously higher than that of model group (P<0.01), all treatment groups could obviously improve UC model rat pathological damage and disease activity index in colon tissue, and Jianpi-Huazhi pill group and Jianpi-Qinghua-Huoxue group showed better efficacy. All treatment groups could inhibit mRNA and protein expressions of TLR-4, MyD88 and NF-κB in colon tissue (P<0.001). Mesalazine group, Jianpi-Huazhi pill group, Jianpi-Qinghua-Daozhi group and Qinghua-Daozhi-Huoxue group were significantly better than Jianpi-Qinghua group and Jianpi-Qinghua-Huoxue group in reducing NF-κB mRNA and protein expressions (P<0.05). All groups could reduce expression of TNF-α in colon tissue (P<0.001), and there was no significant difference between groups (P>0.05). Conclusion: Jianpi- Huazhi pill and its various deconditions may play roles through TLR-4/MyD88/NF-κB pathway. Among them, Coptid-Rhizoma coptifolia, stews woody incense, Fengwei herb and gung ginger may be core drugs of this recipe, which has certain guiding significance for clinical practice. [ABSTRACT FROM AUTHOR]