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Immunohistochemical analysis of PDGFR-α for wound age determination.

Authors :
Zhang, Wei
Ishida, Yuko
Nosaka, Mizuho
Kuninaka, Yumi
Ishigami, Akiko
Yasuda, Haruki
Kofuna, Ayumi
Kamata, Ten
Goto, Utako
Kondo, Toshikazu
Source :
International Journal of Legal Medicine. Jul2024, Vol. 138 Issue 4, p1351-1356. 6p.
Publication Year :
2024

Abstract

Immunohistochemical analysis of platelet-derived growth factor receptor-α (PDGFR-α) was performed on human skin wounds obtained from forensic autopsy cases. Thirty human skin wounds were collected at different post-infliction intervals as follows: Group I, 4 h to 3 days (n = 16); Group II, 4 to 7 days (n = 7); Group III, 9 to 10 days (n = 3); and Group IV, 14 to 20 days (n = 4). Immunopositive reactions for PDGFR-α were not observed in the uninjured human skin specimens. In a semi-quantitative morphometrical analysis, the number of PDGFR-α-positive cells was observed increased in Group II, with the average number of PDGFR-α-positive cells being the highest in Group II. Additionally, in Group II, all specimens showed PDGFR-α-positive cells, with an average number of > 200 cells in five fields of view, suggesting a wound age of 4 to 7 days. Taken together, the immunohistochemical detection of PDGFR-α in human skin wounds can be a useful tool for wound age determination. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09379827
Volume :
138
Issue :
4
Database :
Academic Search Index
Journal :
International Journal of Legal Medicine
Publication Type :
Academic Journal
Accession number :
177777851
Full Text :
https://doi.org/10.1007/s00414-024-03208-0