环境因子对西藏野生手掌参 根际土壤细菌群落结构的影响.

[Objective] The present paper aimed to explore the effects of environmental factors on bacterial community structure in rhizosphere soil of wild Gymnadenia conopsea in Tibet. (Method) Therefore, four sampling sites were selected to collect soil samples, including the abandoned timber factory (FM), Linzhi viewing station (LZ), Linhai viewing station (LH), and the radar station (LD), and the high-throughput sequencing technology was used to analyze the diversity and structure of bacterial community in rhizosphere soil of G. conopsea at different altitudes, and the effects of different environmental factors on the diversity and structure of bacterial community were analyzed. [Result] The total of 32 phyla, 90 classes, 224 orders, 378 families, and 693 genera were detected in all samples. Proteobacteria, Actinobacteria and Acidobacteria were the dominant phyla, Bradyrhizobium and Chryseobacterium were the dominant genera. With the increase of altitude, the diversity and richness of rhizosphere soil bacterial community increased, and the difference between groups was greater than the difference within groups. [Conclusion] Altitude, TK, pH and EC significantly affected the bacterial community composition in rhizosphere soil at phylum level. Three environmental factors, namely TK, TP and altitude, had more obvious effects on bacterial community composition at genus level. Key words: Gymnadenia conopsea; Environmental factor; Rhizosphere soil bacteria; Community diversity; Community structure [ABSTRACT FROM AUTHOR]