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葛花提取物通过激活PPARγ 上调ABCA1 的表达而抑制 THP-1 源性泡沫细胞形成.
- Source :
-
Chinese Journal of Arteriosclerosis . May2024, Vol. 32 Issue 5, p395-401. 7p. - Publication Year :
- 2024
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Abstract
- Aim To explore the effect of Pueraria Lobata Flowers Extract (PFE) on lipid accumulation in macrophage-derived foam cells. Methods The concentration of PFE in THP-1-derived foam cells was screened by MTT, intracellular lipid accumulation was detected by oil red O staining and cholesterol detection kit, intracellular cholesterol efflux levels were detected by cholesterol efflux assay kit, RT-qPCR and Western blot were used to analyze mRNA and protein expression. Results PFE significantly reduced lipid accumulation in THP-1-derived foam cells. PFE did not affect the mRNA expression of CD36, scavenger receptor-AⅠ (SR-AⅠ), sterol regulatory element-binding protein 2 (SREBP2), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), but it could upregulate the mRNA and protein expression levels of ATP-binding cassette transporter A1 (ABCA1) (P<0. 05), and promote the intracellular cholesterol efflux of macrophage-derived foam cells (P<0. 01). PFE could activate the activity of peroxisome proliferator-activated receptor γ (PPARγ) (P<0. 01) and upregulate the mRNA and protein expression levels of PPARγ (P<0. 05). Compared with the PFE control group, the expression of PPARγ and ABCA1 proteins decreased and cholesterol efflux decreased after GW9662 treatment (all P<0. 01). Conclusion PFE could significantly prevent the lipid accumulation in THP-1-derived foam cells and inhibit the formation of foam cells by upregulating ABCA1 expression and cholesterol efflux mediated by PPARγ. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 10073949
- Volume :
- 32
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Chinese Journal of Arteriosclerosis
- Publication Type :
- Academic Journal
- Accession number :
- 177833785
- Full Text :
- https://doi.org/10.20039/j.cnki.1007-3949.2024.05.004