Synthesis, anti-inflammatory, antibacterial, and antioxidant evaluation of novel pyrazole-linked hydrazone derivatives.

• A series of new hydrazones were synthesized with good yield. • Synthesized compounds were evaluated for antibacterial, anti-inflammatory, and anti-oxidant activities. • Upon comparing the synthesized molecules with the commonly prescribed medication Diclofenac sodium, we observed a significant enhancement in anti-inflammatory efficacy. • The synthesized compounds demonstrated a notable inclination for DPPH scavenging in the antioxidant assessment, further highlighting their potential in combating oxidative stress. • In silico molecular docking and dynamic investigations were performed to better understand the binding mechanism of the synthesized hydrazones with the target. Hydrazones, which are azomethine-containing active chemicals with a N(H)N CH group, are extensively researched due to their versatility in pharmacology and ease of synthesis. The synthesized hydrazone compounds were examined by FTIR, UV–visible, EI-MS, 1H NMR, and 13C NMR spectroscopy, and they possessed azomethine linkages. Using the disc diffusion process and minimum inhibitory concentration (MIC) techniques, the compounds were tested for their antibacterial activity. By using the denaturation of bovine serum albumin technique to the synthesized compounds, their anti-inflammatory properties were further examined. Comparing the synthesized molecules to the common medication diclofenac sodium, considerable anti-inflammatory efficacy was observed. Synthesized compounds' antioxidant results revealed a remarkable tendency for DPPH scavenging. Further in silico molecular docking and dynamic studies were evaluated to understand the binding mechanism of the synthesized hydrazones with the target. [Display omitted] [ABSTRACT FROM AUTHOR]