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Mechanical Strain Induces and Increases Vesicular Release Monitored by Microfabricated Stretchable Electrodes.

Authors :
Yan, Jing
Zhang, Fu‐Li
Jin, Kai‐Qi
Li, Jia‐Xin
Wang, Li‐Jun
Fan, Wen‐Ting
Huang, Wei‐Hua
Liu, Yan‐Ling
Source :
Angewandte Chemie International Edition. 7/22/2024, Vol. 63 Issue 30, p1-7. 7p.
Publication Year :
2024

Abstract

Exocytosis involving the fusion of intracellular vesicles with cell membrane, is thought to be modulated by the mechanical cues in the microenvironment. Single‐cell electrochemistry can offer unique information about the quantification and kinetics of exocytotic events; however, the effects of mechanical force on vesicular release have been poorly explored. Herein, we developed a stretchable microelectrode with excellent electrochemical stability under mechanical deformation by microfabrication of functionalized poly(3,4‐ethylenedioxythiophene) conductive ink, which achieved real‐time quantitation of strain‐induced vesicular exocytosis from a single cell for the first time. We found that mechanical strain could cause calcium influx via the activation of Piezo1 channels in chromaffin cell, initiating the vesicular exocytosis process. Interestingly, mechanical strain increases the amount of catecholamines released by accelerating the opening and prolonging the closing of fusion pore during exocytosis. This work is expected to provide revealing insights into the regulatory effects of mechanical stimuli on vesicular exocytosis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14337851
Volume :
63
Issue :
30
Database :
Academic Search Index
Journal :
Angewandte Chemie International Edition
Publication Type :
Academic Journal
Accession number :
178531766
Full Text :
https://doi.org/10.1002/anie.202403241