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A gradient phage-assisted continuous evolution method for screening suppressor tRNAs in Escherichia coli.
- Source :
-
New Biotechnology . Sep2024, Vol. 82, p85-91. 7p. - Publication Year :
- 2024
-
Abstract
- Suppressor tRNAs, notable for their capability of reading through the stop codon while maintaining normal peptide synthesis, are promising in treating diseases caused by premature termination codons (PTC). However, the lack of effective engineering methods for suppressor tRNAs has curtailed their application potential. Here, we introduce a directed evolution technology that employs phage-assisted continuous evolution (PACE), combined with gradient biosensors featuring various PTCs in the M13 gene III. Utilizing this novel methodology, we have successfully evolved tRNATrp (UGG) reading through the UGA stop codon in Escherichia coli. Massively parallel sequencing revealed that these mutations predominantly occurred in the anticodon loop. Finally, two suppressor tRNATrp (UGA) mutants exhibited over fivefold increases in readthrough efficiency. [Display omitted] • A directed evolution technology for suppressor tRNAs was developed in E. coli. • Suppressor tRNA mutations mainly occurred in the anticodon loop. • Two suppressor tRNATrp (UGA) exhibited over fivefold increase in efficiency. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18716784
- Volume :
- 82
- Database :
- Academic Search Index
- Journal :
- New Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 177910052
- Full Text :
- https://doi.org/10.1016/j.nbt.2024.05.005