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Circular RNA circ&_0003609 ameliorates hypertrophied ligamentum flavum by regulating the miR-155/SIRT1 axis.
- Source :
-
Biocell . 2024, Vol. 48 Issue 6, p1001-1008. 8p. - Publication Year :
- 2024
-
Abstract
- Background: Hypertrophy of the ligamentum flavum (HLF) is a common contributor to spinal stenosis which results in significant neurological impairments. Circular RNA (circRNA) circ_0003609 has been linked to HLF; however, the exact mechanism by which it causes this disease is unclear. Methods: Circ_0003609 expressions were regulated in HLF cells by overexpression vectors and RNA interference. Cell proliferation and fibrosis-related gene expression were checked by the Cell Counting Kit-8 (CCK-8) assay and western blotting. CircBank's prediction of the association between miR-155 and circ_0003609 was supported by a dual-luciferase reporter experiment. The function of the miR-155/sirtuin 1 (SIRT1) axis in controlling HLF fibrosis was further examined. Results: Overexpression of circ_0003609 suppressed HLF cell propagation and fibrosis compared to its silencing. It was found that circ_0003609 served as the sponge for miR-155 and that the circ_0003609/miR-155 axis controlled the fibrosis of HLF cells. It was found that circ_0003609 acted as a sponge for miR-155, regulating the fibrosis of HLF cells. Further, miR-155 targets SIRT1, and the miR-155/SIRT1 axis promotes HLF cell fibrosis. Conclusion: Circ_0003609 ameliorates hypertrophied ligamentum flavum (LF) by modulating the miR-155/SIRT1 axis, indicating a potential treatment approach for HLF. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CIRCULAR RNA
*HYPERTROPHY
*SPINAL stenosis
*NEUROLOGY
*CELL proliferation
Subjects
Details
- Language :
- English
- ISSN :
- 03279545
- Volume :
- 48
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Biocell
- Publication Type :
- Academic Journal
- Accession number :
- 177920479
- Full Text :
- https://doi.org/10.32604/biocell.2024.050294