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Identification of different classes of genome instability suppressor genes through analysis of DNA damage response markers.
- Source :
-
G3: Genes | Genomes | Genetics . Jun2024, Vol. 14 Issue 6, p1-14. 14p. - Publication Year :
- 2024
-
Abstract
- Cellular pathways that detect DNA damage are useful for identifying genes that suppress DNA damage, which can cause genome instability and cancer predisposition syndromes when mutated. We identified 199 high-confidence and 530 low-confidence DNA damage-suppressing (DDS) genes in Saccharomyces cerevisiae through a whole-genome screen for mutations inducing Hug1 expression, a focused screen for mutations inducing Ddc2 foci, and data from previous screens for mutations causing Rad52 foci accumulation and Rnr3 induction. We also identified 286 high-confidence and 394 low-confidence diverse genome instability-suppressing (DGIS) genes through a whole-genome screen for mutations resulting in increased gross chromosomal rearrangements and data from previous screens for mutations causing increased genome instability as assessed in a diversity of genome instability assays. Genes that suppress both pathways (DDS+ DGIS+) prevent or repair DNA replication damage and likely include genes preventing collisions between the replication and transcription machineries. DDS+ DGIS− genes, including many transcription-related genes, likely suppress damage that is normally repaired properly or prevent inappropriate signaling, whereas DDS− DGIS+ genes, like PIF1 , do not suppress damage but likely promote its proper, nonmutagenic repair. Thus, induction of DNA damage markers is not a reliable indicator of increased genome instability, and the DDS and DGIS categories define mechanistically distinct groups of genes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21601836
- Volume :
- 14
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- G3: Genes | Genomes | Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 177947343
- Full Text :
- https://doi.org/10.1093/g3journal/jkae064