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A comparative study on the cucurbit[7]uril‐based indicator displacement assay for methyl Viologen. A theoretical and experimental perspective.
- Source :
-
Journal of Physical Organic Chemistry . Jul2024, Vol. 37 Issue 7, p1-11. 11p. - Publication Year :
- 2024
-
Abstract
- One colorimetric and one fluorescent‐indicator displacement assay (IDA) were studied to assess their interaction with methyl viologen (MV), an herbicide commercially known as paraquat. The host–guest complexes formed by cucurbit[7]uril (CB[7]) and the 4‐nitroaniline (NA) or Berberine (Be) dyes function as colorimetric‐ and fluorescent‐IDA for MV, respectively. The CB[7]@NA and CB[7]@Be complexes were characterized by UV–vis, fluorescence spectroscopy, and dynamic simulations. Isothermal titration calorimetry (ITC) experiments and dynamic simulations were employed to obtain thermodynamic data for the formation of the 1:1 inclusion complexes. Finally, NA is proposed as a reference colorimetric dye for determining CB[7]‐binding affinities using competitive host–guest titrations, and the fluorescent‐IDA as the best assay proposed, as it exhibits a high binding constant, a good detection limit of 2.7 × 10−6 mol L‐1, good linearity in the range from 4 × 10−6 mol L‐1 to 3.5 × 10−5 mol L‐1, in aqueous solution. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08943230
- Volume :
- 37
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of Physical Organic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 178021011
- Full Text :
- https://doi.org/10.1002/poc.4581