Navigating the intricacies of RT-qPCR data analysis in gene expression studies.

This document titled "Navigating the intricacies of RT-qPCR data analysis in gene expression studies" is a practical guide for beginners in analyzing data from gene expression studies using quantitative reverse transcription real-time PCR (RT-qPCR). It covers various aspects of data analysis, including the use of controls, melt curves, selection of reference genes, and relative quantification. The document also provides a ready-to-use spreadsheet for relative quantification and emphasizes the importance of careful interpretation of results. It highlights the significance of selecting suitable reference genes for accurate gene expression measurement. The article aims to support researchers in analyzing RT-qPCR data and serves as a reference for molecular biology educators. [Extracted from the article]