补肾健脾活血方对骨质疏松大鼠肝脏脂肪病变的作用.

Objective To explore the hepatic steatosis in osteoporosis (OP) model rats and the effect of the nourishing kidney, invigorating spleen, and promoting blood flow recipe. Methods Twenty-four 3-month-old SPF-grade female rats were optionally allocated into the sham-operation group (n=6) and the mock-up group (n=18). After the ovaries were removed to ascertain the success of modelling, 12 rats in the mock-up were randomly sampled, and the rats were gavaged with alendronate (n=6) and the nourishing kidney, invigorating spleen, and promoting blood flow recipe (n=6) for three months. BMD was detected using DXA. Micro CT was used to observe the bone microstructure. Biochemical tests were performed to detect ALT and AST. Oil-red O staining and HE sections were used to observe the changes in the liver. Immunohistochemistry was performed to observe the changes of glutathione peroxidase 4 (GPX4). Results Compared to those in the sham group, ALT and AST in the mock-up group increased (P<0.05). BMD of the lumbar spine in the model group was lower than that in the sham-operated group (P<0.05). The number of bone trabeculae in the mock-up group was reduced and the bone microstructure was damaged. Fat infiltration in the liver was obvious in the mock-up group, and the hepatic lobes were unclear and the hepatic cords were far from regular. After intervention with the nourishing kidney, invigorating spleen, and promoting blood flow recipe, the fat infiltration was reduced and the hepatic lobes were clearer, and the hepatic cords were regular. Comparing to that in the mock-up group, the expression of GPX4 decreased in the model group (P<0.05). Conclusion Osteoporosis may also lead to fatty lesions in the liver. The nourishing kidney, invigorating spleen, and promoting blood flow recipe has the ability to slow down the fatty lesions in the liver of the rats. GPX4 may be involved in the relationship between osteoporosis and the progression of fatty liver. [ABSTRACT FROM AUTHOR]