基于高通量测序分析滇龙胆 根际土壤微生物群落特征.

[Objective] The paper aimed to provide scientific and effective theoretical basis for understanding the rhizosphere soil microbial community structure, making full use of microbial resources and cultivating Gentiana rigescens Franch. with good quality. [Method] The microbial diversity and community structure of rhizosphere soil of G. rigescens Franch. from different producing areas were compared and analyzed by high-throughput sequencing technology. The correlation between microbial community abundance and soil nutrient content in rhizosphere soil was analyzed in combination with soil physicochemical properties. [Result] There were differences in the diversity and richness of rhizosphere soil microorganisms in different areas, among which the Chaol, Ace and Shannon indices of sample BS2 were the largest. The analysis of community composition showed that the bacteria belonged to 136 phylums, 86 classes, 188 orders, 409 families and 1793 genera. The dominant bacteria of DL2 and LC were Proteobacteria, and their relative abundance was 64.74% and 56.00%, respectively. Acidobacteria were the dominant bacteria in samples DLI, BSI, BS2 and GZ, and their relative abundance was 42.02%, 24. 40%, 41.44% and. 71. 30%, respectively. Fungi of rhizosphere soil belonged to 5 phylums, 28 classes, 63 orders, 124 families and 181 genera. Basidiomycota (42.65%) was the dominant fungus in sample BS2, and Ascomycota (48.50% -88.92%) was the dominant fungus in the other five samples. Correlation analysis of rhizosphere microorganisms and soil physicochemical properties showed that soil pH was significantly positively correlated with the relative abundance of Streptomyces and negatively correlated with Candidatus-Sulfotelmatter. The organic matter content was significantly positively correlated with the relative abundance of both Edaphobacter and Granulicella. Soil available nutrients such as hydrolyzed nitrogen, available phosphorus and available potassium were significantly or extremely significantly positively correlated with the relative abundance of Rhodanobacter. [Conclusion] The origin factors have a certain effect on the microbial diversity and community structure of rhizosphere soil of G. rigescens. Comprehensively, sample BS2 has the highest richness and diversity of rhizosphere soil microorganisms. Proteobacteria, Acidobacteria, Actinobacteria and Chloroflexi are the dominant bacteria in all samples, while Ascomycota and Basidiomycota are the dominant fungal communities. [ABSTRACT FROM AUTHOR]