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Application of 3D bioprinting technology apply to assessing Dangguiniantongtang (DGNT) decoctions in arthritis.
- Source :
-
Chinese Medicine . 7/8/2024, Vol. 19 Issue 1, p1-12. 12p. - Publication Year :
- 2024
-
Abstract
- The aim of this study was to develop a three-dimensional (3D) cell model in order to evaluate the effectiveness of a traditional Chinese medicine decoction in the treatment of arthritis. Chondrocytes (ATDC5) and osteoblasts (MC3T3-E1) were 3D printed separately using methacryloyl gelatin (GelMA) hydrogel bioinks to mimic the natural 3D cell environment. Both cell types showed good biocompatibility in GelMA. Lipopolysaccharide (LPS) was added to the cell models to create inflammation models, which resulted in increased expression of inflammatory factors IL-1β, TNF-α, iNOS, and IL-6, and decreased expression of cell functional genes such as Collagen II (COLII), transcription factor SOX-9 (Sox9), Aggrecan, alkaline phosphatase (ALP), RUNX family transcription factor 2 (Runx2), Collagen I (COLI), Osteopontin (OPN), and bone morphogenetic protein-2 (BMP-2). The created inflammation model was then used to evaluate the effectiveness of Dangguiniantongtang (DGNT) decoctions. The results showed that DGNT reduced the expression of inflammatory factors and increased the expression of functional genes in the cell model. In summary, this study established a 3D cell model to assess the effectiveness of traditional Chinese medicine (TCM) decoctions, characterized the gene expression profile of the inflammatory state model, and provided a practical reference for future research on TCM efficacy evaluation for arthritis treatment. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DRUG therapy for arthritis
*CHINESE medicine
*BIOLOGICAL models
*OSTEOBLASTS
*RESEARCH funding
*HERBAL medicine
*POLYMERASE chain reaction
*TRANSCRIPTION factors
*ALKALINE phosphatase
*PLANT extracts
*CELL lines
*BIOMEDICAL materials
*GENE expression
*BONE morphogenetic proteins
*EXPERIMENTAL design
*CARTILAGE cells
*LIPOPOLYSACCHARIDES
*DRUG efficacy
*THREE-dimensional printing
*COLLAGEN
*INTERLEUKINS
Subjects
Details
- Language :
- English
- ISSN :
- 17498546
- Volume :
- 19
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 178332408
- Full Text :
- https://doi.org/10.1186/s13020-024-00948-4