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TRPV4 modulation participates in paraoxon-induced brain injury via NMDA and NLRP3 regulation.
- Source :
-
Brain Injury . 2024, Vol. 38 Issue 10, p848-857. 10p. - Publication Year :
- 2024
-
Abstract
- Organophosphorus pesticide poisoning can lead to severe brain damage, but the specific mechanisms involved are not fully understood. Our research aims to elucidate the function of the TRPV4 ion channel in the development of brain injury induced by paraoxon (POX). In vivo, we examined the survival rate, behavioral seizures, histopathological alterations, NMDA receptor phosphorylation, as well as the expression of the NLRP3-ASC-caspase-1 complex and downstream inflammatory factors in the POX poisoning model following intervention with the TRPV4 antagonist GSK2193874. In vitro, we investigated the effects of GSK2193874 on NMDA-induced inward current, cell viability, cell death rate, and Ca2+ accumulation in primary hippocampal neurons. The treatment with the TRPV4 antagonist increased the survival rate, suppressed the status epilepticus, improved pathological damage, and reduced the phosphorylation level of NMDA receptors after POX exposure. Additionally, it inhibited the upregulation of NLRP3 inflammasome and inflammatory cytokines expression after POX exposure. Moreover, the TRPV4 antagonist corrected the NMDA-induced increase in inward current and cell death rate, decrease in cell viability, and Ca2+ accumulation. TRPV4 participates in the mechanisms of brain injury induced by POX exposure through NMDA-mediated excitotoxicity and NLRP3-mediated inflammatory response. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CALCIUM metabolism
*IN vitro studies
*T-test (Statistics)
*RESEARCH funding
*PHOSPHORYLATION
*CELLULAR signal transduction
*IN vivo studies
*CYTOCHEMISTRY
*PESTICIDES
*RATS
*GENE expression
*ORGANOPHOSPHORUS compounds
*ANIMAL experimentation
*ONE-way analysis of variance
*SEIZURES (Medicine)
*CELL death
*BRAIN injuries
*DATA analysis software
*INFLAMMATION
*CYTOKINES
*CELL survival
*ION channels
*CELL receptors
*SIGNAL peptides
*DISEASE risk factors
Subjects
Details
- Language :
- English
- ISSN :
- 02699052
- Volume :
- 38
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Brain Injury
- Publication Type :
- Academic Journal
- Accession number :
- 178419202
- Full Text :
- https://doi.org/10.1080/02699052.2024.2351104