益生菌灌服/健康大鼠肠道微生物群移植 对大鼠妊娠期糖尿病的治疗作用及其机制.

Objective To observe the therapeutic effects of probiotics gavage/healthy rat intestinal microbiota transplantation on gestational diabetes mellitus (GDM) in rats and to investigate their mechanisms. Methods Seventy female and 35 male Sprague-Dawley (SD) rats were used, with a 2: 1 ratio for mating. After successful mating, 10 female rats were randomly selected as the control group (marked as day 0). The remaining 60 female rats were injected intraperitoneally with streptozotocin to establish the GDM models. Upon successful modeling (random blood glucose ≥ 16.7 mmol/L), 40 rats were divided into four groups, with 10 rats in each. On the 5th day, rats in the first group received a gavage of 359. 708 mg/kg probiotics supplement, rats in the second group received a gavage of 1 mL/100g of healthy rat fecal microbiota suspension, rats in the third group received both 359.708 mg/kg probiotics supplement and 1 mL/100g healthy rat fecal microbiota suspension, and rats in the fourth group, along with the control group, received 10 mL/kg PBS buffer gavage. Each group was gavaged once daily for 14 consecutive days. On the 19th day, an oral glucose tolerance test was performed to measure blood glucose levels. On the 20th day, colonic contents and tissues were collected after anesthesia to measure the short-chain fatty acids (SCFAs) (acetate, propionate, butyrate, and isobutyrate) content using the GC-2010 gas chromatograph. The specific G protein-coupled free fatty acid receptor (GPR43) and glucagon-like peptide-1 (GLP-1) mRNA and protein levels were detected using RT-qPCR and Western blotting. Results Compared with the control group, the GDM rats on the 20th day exhibited lower glucose tolerance, reduced levels of acetate, butyrate, and isobutyr- ate in colonic contents, and lower relative expression levels of GPR43 mRNA, GLP-1 mRNA, and protein in colonic tissues of the fourth group (all P<0.05). Compared with the fourth group, the first, second, and third groups showed higher glucose tolerance and increased relative expression levels of GPR43 mRNA, GLP-1 mRNA, and protein in the colonic tissues on the 20th day (all P<0.05). Conclusions Probioties gavage and healthy rat intestinal microbiota transplantation can improve GDM in rats. This improvement may be achieved by increasing SCFA content in colonic contents and enhaneing the expression of GPR43, GLP-1 mRNA, and protein in colonic tissues, thus ameliorating GDM in rats. [ABSTRACT FROM AUTHOR]