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The cellulose synthase gene PrCESA10 is involved in cellulose biosynthesis in developing tracheids of the gymnosperm Pinus radiata
- Source :
-
Gene . May2005, Vol. 350 Issue 2, p107-116. 10p. - Publication Year :
- 2005
-
Abstract
- Abstract: One full length (PrCESA10) and seven other cDNA clones (PrCESA2, 3, 5–8, 11) encoding cellulose synthases (CESAs) were isolated from the coniferous gymnosperm Pinus radiata. PrCESA10 encodes a protein predicted to contain the same domains and regions as angiosperm CESA genes: a zinc finger domain, a hypervariable region 1 (HVR1), a plant-conserved region (CR-P), a class-specific region or hypervariable region 2 (HVR2), in addition to the four conserved domains U1–U4 that are characteristic of the family 2 processive β-glycosyltransferases. The P. radiata protein is also predicted to contain eight transmembrane domains. The zinc finger domain, the CR-P and the C-terminal portion of the proteins, are highly conserved between P. radiata and the nearest angiosperm CESA protein from Solanum tuberosum. Reverse transcriptase-PCR showed that all the P. radiata genes were expressed in all organs tested, although to different extents. In situ hybridization studies with PrCESA10 in stems of 2- and 12-month-old seedlings showed that it was expressed in the secondary xylem in the two-to-three most recently developed tracheids, which were laying down secondary cell walls. [Copyright &y& Elsevier]
- Subjects :
- *PINUS radiata
*HEREDITY
*DNA polymerases
*REVERSE transcriptase
Subjects
Details
- Language :
- English
- ISSN :
- 03781119
- Volume :
- 350
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 17852233
- Full Text :
- https://doi.org/10.1016/j.gene.2005.02.018