黄蜀葵花总黄酮对肾脏葡萄糖重吸收 影响的实验研究.

Objective To explore the effect of total flavones of Abelmoschus manihot (TFA) on renal glucose reabsorption. Methods A total of 24 C57BL/6 mice were assigned to control, high fat diet (HFD), and HFD+TFA groups using random number table method, 8 mice in each group. In vitro, HK2 cells were treated with palmitic acid (PA). The cells were divided into 3 groups: control group, PA group, and PA+TFA group. Oil red O staining was used to evaluate lipid accumulation. Immunofluorescence staining, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blotting were used to evaluate glucose uptake and sodium glucose cotransporter 2 (SGLT2) expression. One-way analysis of variance (ANOVA) was used for comparison among groups. Results Massive lipid deposition in renal tubules was observed in HFD group, whereas no lipid deposition was found in the control group. Compared with HFD group, red-stained lipid droplets were significantly decreased in HFD+TFA group (P<0.001). The immunofluorescence results showed that SGLT2 protein in renal cortex tissue was dectected in three groups. Compared with the control group, SGLT2 expressions were significantly increased in HFD group and HFD+TFA group (P<0.05). However, SGLT2 expression in HFD+TFA group was lower than HFD group (P=0.049). The qRT-PCR results showed that SGLT2 mRNA in HFD group was significantly higher than that in the control group (P<0.001), while decreased after TFA intervention. In addition, treatment with TFA resulted in significant amelioration of lipid accumulation induced by PA in HK2 cells. Accompanied with improvement of lipid deposition, SGLT2 expression and glucose uptake were attenuated (P<0.05). Conclusions TFA can ameliorate renal lipid deposition, thereby inhibiting glucose reabsorption. Its renal protective effect may be related to its role in inhibiting SGLT2 expression. [ABSTRACT FROM AUTHOR]