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Designer cellular spheroids with DNA origami for drug screening.

Authors :
Jiayi Wei
Yueyang Sun
Heming Wang
Tong Zhu
Li Li
Ying Zhou
Quan Liu
Zhen Dai
Wenjuan Li
Taihua Yang
Bingmei Wang
Changfeng Zhu
Xizhong Shen
Qunyan Yao
Guangqi Song
Yicheng Zhao
Hao Pei
Source :
Science Advances. 7/19/2024, Vol. 10 Issue 29, p1-13. 13p.
Publication Year :
2024

Abstract

Current in vitro models struggle to balance the complexity of human diseases with suitability for large-scale drug tests. While 3D cultures simulate human tissues, they lack cellular intricacy, and integrating these models with high-throughput drug screening remains a challenge. Here, we introduce a method that uses self-assembling nucleic acid nanostructures decorated living cells, termed NACs, to create spheroids with a customizable 3D layout. To demonstrate its uniqueness, our method effectively creates designer 3D spheroids by combining parenchymal cells, stromal cells, and immune cells, leading to heightened physiological relevance and detailed modeling of complex chronic diseases and immune-stromal interactions. Our approach achieves a high level of biological fidelity while being standardized and straightforward to construct with the potential for large-scale drug discovery applications. By merging the precision of DNA nanotechnology with advanced cell culture techniques, we are streamlining human-centric models, striking a balance between complexity and standardization, to boost drug screening efficiency. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23752548
Volume :
10
Issue :
29
Database :
Academic Search Index
Journal :
Science Advances
Publication Type :
Academic Journal
Accession number :
178581393
Full Text :
https://doi.org/10.1126/sciadv.ado9880