巴戟天多糖对文昌鸡肠道形态、 肠道免疫屏障功能和盲肠微生物的影响.

The experiment aims to study the effects of Morinda officinalis polysaccharide (MOP) added to the feed on the intestinal morphology, intestinal immune barrier function, and cecal microbiota of Wenchang chickens. Six hundred healthy female Wenchang chickens aged six days old were randomly divided into five groups, with eight replicates per group and 15 chickens per replicate. The control group was fed a basic diet, while each experimental group added 250, 500, 1 000, 2 000 mg/kg of MOP to the basic diet. The experiment lasted for 40 days. The results showed that compared with the control group, the villus height/crypt depth of the duodenum and jejunum in each experimental group were significantly increased (P<0.05), and the 500, 1 000, 2 000 mg/kg MOP groups significantly improved the villus height/crypt depth of the ileum (P<0.05). The relative expression of Occludin mRNA in the duodenum in the 250, 500, 1 000 mg/kg MOP groups was significantly increased (P<0.05), and the relative expression of TNF-α mRNA in the duodenum in the 1 000, 2 000 mg/kg MOP groups was significantly reduced (P<0.05). The number of observed species and the Ace index in the cecum in the 250, 500 mg/kg MOP groups were significantly increased (P<0.05). The Shannon index in all the experimental groups was significantly increased (P<0.05), the Simpson index in the 2 000 mg/kg MOP group was significantly increased (P<0.05), the Chao1 index in the 250, 500, 1 000 mg/kg MOP groups was significantly increased (P<0.05). The relative abundance of Campylobacter in the cecum in all the experimental groups was significantly reduced (P<0.05), the 1 000, 2 000 mg/kg MOP groups significantly reduced the relative abundance of the Bifidobacterium in the cecum (P<0.05), and significantly increased the relative abundance of the beneficial bacteria Parabacteroides (P<0.05). The addition of 2 000 mg/kg MOP in the feed significantly reduced the relative abundance of Streptococcus (P<0.05). The 250, 2 000 mg/kg MOP groups significantly reduced the relative abundance of Bacteroides (P<0.05), and significantly increased the relative abundance of the CHKCI001 genus (P<0.05). The study indicates that the addition of an appropriate amount of MOP to the feed of Wenchang chickens can improve intestinal mucosal morphology, increase the expression of the tight junction protein Occludin mRNA, reduce the expression of the inflammatory factor TNF-α mRNA, increase the Alpha-diversity and evenness of cecal microbiota, increase the content of beneficial bacteria in the intestine, and promote intestinal health. The appropriate amount of MOP is 500 mg/kg. [ABSTRACT FROM AUTHOR]