Back to Search
Start Over
Development and evaluation of immunological effects of a DNA vaccine encoding phosphoketolase family protein against Nocardia seriolae in hybrid snakehead.
- Source :
-
Fish & Shellfish Immunology . Sep2024, Vol. 152, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- Fish nocardiosis is a chronic disease mainly caused by Nocardia seriolae , which occurs in a variety of economically cultured freshwater and marine fish. Studies have shown that DNA vaccine is an effective treatment to protect fish from bacterial infection. In our previous experiment, an in vivo -induced gene of N. seriolae , encoding phosphoketolase (PK) family protein, was identified by in vivo -induced antigen technology. In the present study, the antigenic gene encoding PK family protein was analyzed by bioinformatics and further inserted into the eukaryotic expression vector pcDNA3.1-myc-his-A for DNA vaccine development. The immunological effects of pcDNA-PK DNA vaccine were assessed in hybrid snakehead (Channa maculata ♀ × Channa argus ♂), showing induction in several serum enzyme activity parameters (including LZM, SOD, ACP and AKP), increasing in specific-antibody IgM levels, as well as up-regulation in six immune-related genes (CD4 , CD8α , TNFα , IL-1β , MHCIα and MHCIIα). Moreover, an immune-protection with a relative survival rate was provided at 53.82 % following artificial challenge with N. seriolae in vaccinated fish in comparison to the control group. In summary, these results indicate that pcDNA-PK DNA vaccine could boost strong immune responses in hybrid snakehead and show preferably protective efficacy against N. seriolae , which may be applied in aquaculture to control fish nocardiosis. • An in vivo -induced antigenic gene, PK of Nocardia seriolae was used for DNA vaccine development. • The pcDNA-PK DNA vaccine can induce humoral as well as cellular immune responses in hybrid snakehead. • There was relative percentage survival at 53.82 % of pcDNA-PK DNA vaccine against N. seriolae. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA vaccines
*MARINE fishes
*VACCINE effectiveness
*GENE expression
*NOCARDIOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 10504648
- Volume :
- 152
- Database :
- Academic Search Index
- Journal :
- Fish & Shellfish Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 178940317
- Full Text :
- https://doi.org/10.1016/j.fsi.2024.109773