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Spatiotemporal transcriptome atlas reveals gene regulatory patterns during the organogenesis of the rapid growing bamboo shoots.

Authors :
Guo, Jing
Luo, Dan
Chen, Yamao
Li, Fengjiao
Gong, Jiajia
Yu, Fen
Zhang, Wengen
Qi, Ji
Guo, Chunce
Source :
New Phytologist. Nov2024, Vol. 244 Issue 3, p1057-1073. 17p.
Publication Year :
2024

Abstract

Summary: Bamboo with its remarkable growth rate and economic significance, offers an ideal system to investigate the molecular basis of organogenesis in rapidly growing plants, particular in monocots, where gene regulatory networks governing the maintenance and differentiation of shoot apical and intercalary meristems remain a subject of controversy.We employed both spatial and single‐nucleus transcriptome sequencing on 10× platform to precisely dissect the gene functions in various tissues and early developmental stages of bamboo shoots. Our comprehensive analysis reveals distinct cell trajectories during shoot development, uncovering critical genes and pathways involved in procambium differentiation, intercalary meristem formation, and vascular tissue development.Spatial and temporal expression patterns of key regulatory genes, particularly those related to hormone signaling and lipid metabolism, strongly support the hypothesis that intercalary meristem origin from surrounded parenchyma cells. Specific gene expressions in intercalary meristem exhibit regular and dispersed distribution pattern, offering clues for understanding the intricate molecular mechanisms that drive the rapid growth of bamboo shoots.The single‐nucleus and spatial transcriptome analysis reveal a comprehensive landscape of gene activity, enhancing the understanding of the molecular architecture of organogenesis and providing valuable resources for future genomic and genetic studies relying on identities of specific cell types. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0028646X
Volume :
244
Issue :
3
Database :
Academic Search Index
Journal :
New Phytologist
Publication Type :
Academic Journal
Accession number :
180109765
Full Text :
https://doi.org/10.1111/nph.20059