Expression of miR-34c and miR-29b in peripheral blood of patients with sensorineural deafness and clinical significance.

Objective To investigate the expression and clinical significance of miR-34c and miR-29b in peripheral blood of patients with sensorineural hearing loss. Methods 140 patients with neurological deafness admitted to our hospital from June 2020 to June 2023 were included in the study group, and 40 healthy subjects were included in the control group during the same period. SNHL patients were categorized into 63 cases in the mildly deaf group, 42 cases in the moderately deaf group, and 35 cases in the severely deaf group according to their hearing impairment. The expressions of miR-34c and miR-29b were detected by qRT-PCR, and VEGF, oxidative stress (TAC, SOD, MDA), NO and Cx26 were detected. Correlation analysis was performed by Pearson test, independent risk factors for SNHL severity were calculated by logistics regression algorithm, and the predictive value of miR-34c and miR-29b on the severity of SNHL patients was evaluated by ROC curve. Results Comparing the levels of NO, TAC, SOD, and Cx26 among the four groups, severe deafness group < moderate deafness group < mild deafness group < control group (P < 0.05); comparing the levels of VEGF and MDA, severe deafness group > moderate deafness group > mild deafness group > control group (P < 0.05). Comparison of miR-34c mRNA and miR-29b mRNA expression levels among the four groups, severe deafness group > moderate deafness group > mild deafness group > control group (P < 0.05). Pearson's test showed that SNHL patients' peripheral blood miR-34c and miR-29b were positively correlated with VEGF and MDA, and were negatively correlated with NO, TAC, SOD and Cx26 (P < 0.05). negative correlation (P < 0.05). Multifactorial logistic regression analysis showed that VEGF, MDA, NO, Cx26, TAC, SOD, miR-34c, miR-29b were independent factors affecting the severity of SNHL. Conclusion miR-34c and miR-29b are overexpressed in SNHL patients and can be used as serum markers for predictive assessment of disease severity in SNHL patients. [ABSTRACT FROM AUTHOR]