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Preparation and chromatographic evaluation of hydrophilic polymer brushes grafted-silica with post modification of silicon/carbon dots as a green liquid chromatography stationary phase.

Authors :
Bo, Chunmiao
Tang, Xiaofan
Li, Yan
Li, Yinhai
Zhao, Weilong
Guo, Shengwei
Source :
Microchimica Acta. Aug2024, Vol. 191 Issue 8, p1-14. 14p.
Publication Year :
2024

Abstract

Polar stationary phases were prepared by grafting hydrophilic acrylamide (Am) polymer brushes with post modification of carbon dots (CDs) and silicon dots (SiDs) onto SiO2 particles. The prepared stationary phases, SiO2-PAm-CDs, SiO2-PAm-CDs/SiDs, and SiO2-PAm-SiDs, were packed as chromatographic columns, respectively. Using nucleic bases, organic acids, and β-agonists as target substances to investigate the influence of chromatographic conditions on retention and separation, the packed columns showed the partitioning and adsorption of mixed retention behavior in hydrophilic interaction liquid chromatography mode and successfully separated the polar compounds. Most importantly, under per aqueous liquid chromatography mode (using 100% water as mobile phase), those columns still had good separation ability toward nucleic bases, β-agonist, and organic acids. Because AM is a temperature-sensitive monomer, the resulting van't Hoff curves exhibited a nonlinear relationship, having temperature-responsive chromatographic characteristic under pure water separation. Hence, building on temperature-sensitive characteristics and pure water of separation conditions, the separation selectivity toward hydrophilic compounds greatly improved. Compared with the commercial hydrophilic columns, the efficiency of our developed column had the superior ability in separation and detection of betaine in Goji berry with the enhanced resolution achieved in the proposed green separation method (just using pure water as mobile phase). [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00263672
Volume :
191
Issue :
8
Database :
Academic Search Index
Journal :
Microchimica Acta
Publication Type :
Academic Journal
Accession number :
179067872
Full Text :
https://doi.org/10.1007/s00604-024-06575-y