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Targeted Protein Fate Modulating Functional Microunits Promotes Intervertebral Fusion.

Authors :
Zheng, Jiancheng
Zhao, Jian
Li, Cuidi
Zhang, Fangke
Saiding, Qimanguli
Zhang, Xingkai
Wang, Guojun
Qi, Jin
Cui, Wenguo
Deng, Lianfu
Source :
Small Methods. Aug2024, Vol. 8 Issue 8, p1-15. 15p.
Publication Year :
2024

Abstract

Stable regulation of protein fate is a prerequisite for successful bone tissue repair. As a ubiquitin‐specific protease (USP), USP26 can stabilize the protein fate of β‐catenin to promote the osteogenic activity of mesenchymal cells (BMSCs) and significantly increased bone regeneration in bone defects in aged mice. However, direct transfection of Usp26 in vivo is inefficient. Therefore, improving the efficient expression of USP26 in target cells is the key to promoting bone tissue repair. Herein, 3D printing combined with microfluidic technology is applied to construct a functional microunit (protein fate regulating functional microunit, denoted as PFFM), which includes GelMA microspheres loaded with BMSCs overexpressing Usp26 and seeded into PCL 3D printing scaffolds. The PFFM provides a microenvironment for BMSCs, significantly promotes adhesion, and ensures cell activity and Usp26 supplementation that stabilizes β‐catenin protein significantly facilitates BMSCs to express osteogenic phenotypes. In vivo experiments have shown that PFFM effectively accelerates intervertebral bone fusion. Therefore, PFFM can provide new ideas and alternatives for using USP26 for intervertebral fusion and other hard‐to‐repair bone defect diseases and is expected to provide clinical translational potential in future treatments. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
23669608
Volume :
8
Issue :
8
Database :
Academic Search Index
Journal :
Small Methods
Publication Type :
Academic Journal
Accession number :
179071559
Full Text :
https://doi.org/10.1002/smtd.202301375