甘露寡糖上调 PIgR 增强肉鸡肠道免疫屏障.

In order to investigate the effect of mannan oligosaccharides on the intestinal barrier of broilers, a total of 540 1-day-old healthy white-feathered broilers were selected for the experiment. Broilers were raised to 29 days old and randomly divided into three groups based on the principle of similar body weight with half male and half female: model control group, MOS health group, and ciprofloxacin lactate health group. Each group had 6 replicates, with 30 replicates each, and the experimental period was 10 d. At the age of 30 d, broilers in the model control group, MOS health group, and ciprofloxacin lactate health group were injected into the pectoral muscle with 8.37×109 CFU/mL Escherichia coli bacterial solution was used for disinfection. Afterwards, drinking water, 0.05 g/L MOS solution, and 60 mg/L ciprofloxacin lactate were administered separately for 10 days. Weigh the broilers after all experiments are completed, blood was collected for serum biochemical indicators, organ indices were measured from various segments of the small intestine, and the expression of key genes in the intestinal barrier of broilers was detected by RT qPCR. The results showed that the body weight and organ index of broilers showed no significant differences among the model control group, MOS health group, and ciprofloxacin lactate health group (P>0.05). Compared with the model control group and the ciprofloxacin lactate health group, the MOS health group broiler serum γ-GGT content significantly increased (P<0.05). Compared with the model control group, the MOS health group showed an increase in PIgR in the duodenum of broiler chickens (P<0.05); compared with the ciprofloxacin lactate health group, the MOS health group showed an increasing trend in the expression of PIgR in the jejunum of broiler chickens (P=0.077). In summary, adding MOS to drinking water increases serum levels γ-GGT upregulates the expression of PIgR in the immune barrier of the duodenum and jejunum of broiler chickens, enhances the intestinal immune barrier of broiler chickens, maintains intestinal health, and thereby improves the immune capacity of the body, with a more significant effect compared to ciprofloxacin lactate. [ABSTRACT FROM AUTHOR]