Ultrasound-Assisted Extraction of Anti-Tyrosinase Activity from Tomatoes.

The high concentration of active substances in tomato extraction is correlated with its anti-tyrosinase and antioxidant effect activity. We established the optimum ultrasound extraction conditions for maximizing the yield of active substances. The four important factors (ethanol concentration, extraction time, extraction temperature, and solid-liquid ratio) that greatly affected ultrasonic extraction were selected to set up a single-factor test and investigate the effects of inhibitory activity on tyrosinase. According to the result of the single-factor test, the orthogonal experiment was conducted to select the optimum process parameter of extracting a total active substance from tomatoes by the alcohol extraction method. The factors affecting the extraction of active substances from tomatoes by an ultrasonic assistant method in order were as follows: ethanol concentration > solid-liquid ratio > extraction temperature > extraction time. The optimum process condition from the orthogonal experiment was as follows: ethanol concentration of 80%, solid-liquid ratio of 1:6, extraction temperature of 50°C, and extraction time of 50 min, under which the average anti-tyrosinase activity of active substances was 32.77% through the tyrosinase inhibition assay. [ABSTRACT FROM AUTHOR]