Neuroprotection mediated by prolactin against streptozotocin injury in brain rat areas.

[Display omitted] • Prolactin protects against streptozotocin-mediated neurodegeneration. • Streptozotocin damage induces neuronal death in cortex, and hippocampus. • Streptozotocin damage increases anxiety in rats. • Prolactin reduces anxiety in streptozotocin-treated rats. Prolactin has been recognized as neuroprotective hormone against various types of neuronal damage. This study was aimed to determine if prolactin protects against streptozotocin injury. A series of experiments were performed to determine neuronal survival by counting total neurons in medial hippocampus cortex and cerebellum. Astrogliosis was determined by immunofluorescence assays using GFAP, and behavioral improvement by prolactin after neuronal damage was determined by open-field and light–dark box tests. Results demonstrated that prolactin induced significant neuronal survival in both the hippocampus and cortex, but not in the cerebellum. No increase in astrogliosis was identified, but a significant reduction in anxiety levels was observed. Overall data indicate that prolactin may protect against a complex form of cell damage including oxidant stress and metabolic disruption by streptozotocin. Prolactin may be helpful strategy in the treatment of neuronal damage in neurological diseases. [ABSTRACT FROM AUTHOR]